Biología celular

ADHESION & MOTILITY
  1. Cell migration assay
  2. 3D assay in matrigel
  3. Carbohydrate-specific adhesion of intact cells to resolved glycolipids on TLC plates
  4. Cell adhesion assay protocol
  5. Cell migration, chemotaxis and invasion assay protocol
  6. Dynamic flow assay in a parallel plate flow chamber
  7. Hanging drop aggregation assay
  8. Measurement of cell adhesion under static conditions
  9. Microtubule (MT)/Organelle motility assays
  10. Motility assay
 
ANGIOGENIC ASSAY
  1. Tube formation assay
  2. Matrigel angiogenesis assay
  3. Vascular permeability assay
 
APOPTOSIS
  1. A rapid & quantitative method for detecting apoptosis by FACS in transiently transfected cells
  2. Cell death detection in xenopus embryos by ELISA
 
CELL CULTURE
  1. Tissue culture methods
  2. ECACC handbook – Fundamental techniques in cell culture
  3. Introduction to animal cell culture
  4. Cell culture guidelines
  5. General cell culture methods
 
CELL CYCLE
  1. Cell cycle
  2. H-thymidine uptake by cultured cells
  3. Timing of cycles
 
CELL GROWTH & CYTOTOXITY
  1. Cell proliferation assay
  2. 3H-thymidine uptake by cultured cells
  3. Cr release cytotoxicity assay
  4. Cytotoxicity assays protocol
  5. Determination of lC50
  6. Timing of cycles
 
CELL INVASION ASSAY
  1. Cell migration, chemotaxis and invasion assay
  2. Invasion assay
  3. Matrigel invasion assay
 
CELL PARTS & ORGANELLES
  1. Em observations of microsomes
  2. Lysosome isolation in isotonic sucrose
  3. Preparation of cytoplasmic extracts
  4. Preparation of rat liver cell cytosol
  5. Preparation of tubulin
 
CELL SEPARATION
  1. T and B cell isolation
 
CELL SYNCHRONIZATION
  1. Metaphase block for cell synchronization
 
CHROMOSOME
  1. Chromatin electrophoresis
  2. Extraction of chromatin
  3. G-banding for analysing mouse chromosomal rearrangements
  4. Karyotype analysis
  5. Mitotic chrosomes from mouse peripheral blood
 
CYTOLOGY
  1. Cytology of mammalian blood
 
FLOW CYTOMETRY (FCM)
  1. FACS troubleshootings
  2. FCM of leukocytes in whole blood
  3. Fluorescein labeling
  4. Phycoerythrin labeling
  5. Protein (antibody) fluorescent labeling
  6. Yeast cell cycle analysis by flow cytometry
  7. Yeast prep for FACS analysis
 
SENESCENCE ASSAY
  1. Cell staining for senescence-associated beta-galactosidase (SA-beta-gal) activity
 
SIGNAL TRANSDUCTION
  1. Pheromone hal assay
  2. SAPK/Jun kinase assays
  3. T-cell activation using mAb to CD3
 
STEM CELLS
  1. Human embryonic stem cell protocols
  2. Stem cells basics

 


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Seguiremos ofreciendo un espacio para todos aquellos que quieran participar y colaborar en esta cruzada educativa, porque tenemos muy claro que estaremos constantemente: “Aprendiendo de los Animales de Laboratorio”.

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